high sensitivity human elisa kits  (R&D Systems)

Journal: Nature Communications

Article Title: Repeat controlled human Plasmodium falciparum infections delay bloodstream patency and reduce symptoms

doi: 10.1038/s41467-024-49041-2

Figure Lengend Snippet: Plasma cytokine levels were measured by multiplex bead analysis for each participant (CHMI 1 n = 8, CHMI 2 n = 8, CHMI 3 n = 8, CHMI 4 n = 5) on D1 before exposure to Pf-infected mosquitoes, D6, D8, day of treatment (DRx) and 7 days after treatment (DRx+7) for each CHMI. A The heatmap of the log of the average ratio of the cytokine concentration (pg/ml) on the indicated day of the CHMI to the baseline concentration following the mock challenge for all participants is shown. B Comparison of the parasitemia (parasites/ml) measured by quantitative PCR (qPCR) of Pf18s rRNA, the ratio of the plasma cytokine levels to the mock CHMI baseline quantified using a multiplex bead assay, and maximal temperature (°C) measured on the day each participant experienced their first and second peak of >3.3 parasites/µl ( n = 6). Data are presented as mean values +/− standard error of the mean (SEM). Statistical differences were tested using Sidak’s multiple comparisons test and the P value is two-sided. Source data are provided as a file.

Article Snippet: IFNγ, IL-10, IL-6, TNF alpha, IL-8, IL-12, IL-1 beta, IL-4 levels were measured using the human high sensitivity cytokine premixed bead kit A (R&D Systems), while standard premixed bead kits (R&D Systems) were used for the rest.

Techniques: Clinical Proteomics, Multiplex Assay, Infection, Concentration Assay, Comparison, Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular Sciences

Article Title: The Severity of Diabetic Retinopathy Corresponds with Corneal Nerve Alterations and Ocular Discomfort of the Patient

doi: 10.3390/ijms25116072

Figure Lengend Snippet: Tear film cytokine levels among DR patients and control subjects (pg/mL). Statistically significant values are shown in bold.

Article Snippet: The concentrations of NGF-β, BDNF, NT-4, and inflammatory mediators (TNF-α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 p70, GM-CSF, and IFN-γ) in tear fluids were measured by multiplex fluorescent bead-based immunoassays (Luminex Corporation, Austin, TX, United States) using commercial R&D Systems Luminex Human Discovery Assay (LXSAHM) and High Sensitivity Cytokine Premixed Kit A Performance Assay (FCSTM09; R&D Systems, Minneapolis, MN, United States).

Techniques: Control